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KMID : 0613820130230101199
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Journal of Life Science
2013 Volume.23 No. 10 p.1199 ~ p.1208
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Functional Analysis of Fibroblastic Reticular Cells Derived from Mouse Lymph Node via Bidirectional Crosstalk with T Cells
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Park Sung-Hee
Lee Jong-Hwan
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Abstract
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Fibroblastic reticular cells (FRCs) form the structural backbone of the T zone provide a guidance path for immigrating T cells in the lymph node (LN). FRCs may contribute directly to developing T-cell biology in the LN and allow analyses of fundamental aspects of FRC biology related to T cells. FRCs inhibited T-cell apoptosis, and FRC culture supernatants strongly induced the expression of Bcl-xL in T cells against doxorubicin. Coculture of FRC and T cells resulted in rearrangements of the actin cytoskeleton, as well as global changes in the morphology of the FRCs. In addition, when cocultured, the T cells adhered to the FRC monolayer, and the membrane intercellular adhesion molecule (ICAM)-1 was slightly increased by day-dependent manner. In contrast, the expression of soluble ICAM-1 was dramatically increased in a day-dependent manner. Several chemokines, such as CCL5, CXCL1, CXCL5, CXCL16, CCL8, CXCL13, and ICAM-1, and MMPs were expressed in FRCs sensed by tumor necrosis factor (TNF) families. Nuclear factor kappa B (NFκB)-RelA of the NFκB canonical pathway was translocated into FRC nuclear by TNFα. In contrast, p52 proteolyzed from p100, a counterpart of RelB of the noncanonical NFκB pathway, accumulated in the peripheral FRC nucleus by agonistic anti-LTβR antibody. In summary, we propose a model in which FRCs engage in bidirectional crosstalk to increase the efficiency of T-cell biology. This cooperative feedback loop may help to maintain tissue integrity and function during immune responses.
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KEYWORD
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Agonistic anti-LTβR antibody, FRC, NFκB, T cell, TNFα
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